abstract：:Vascular smooth muscle cell (VSMC) proliferation plays a key role in neointimal hyperplasia and restenosis. Here we report the role of the microRNA miR-142-5p and its downstream target genes on the proliferation of cultured VSMCs. miR-142-5p promoted VSMC proliferation by down-regulating B cell translocation gene 3 (B...

journal_title：FEBS letters

authors： Kee HJ,Park S,Kwon JS,Choe N,Ahn Y,Kook H,Jeong MH

更新日期：2013-08-02 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' term...

journal_title：FEBS letters

authors： Aoki K,Adachi S,Homoto M,Kusano H,Koike K,Natsume T

更新日期：2013-07-11 00:00:00

abstract：:Oil crops are in increasing demand both for food and as renewable sources of chemicals. It is therefore vital to understand how oil accumulation is regulated. Different ways of obtaining such information are discussed with an emphasis on metabolic control analysis. The usefulness of the latter has been well-illustrate...

journal_title：FEBS letters

authors： Harwood JL,Guschina IA

更新日期：2013-06-27 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...

journal_title：FEBS letters

authors： Demeshkina N,Jenner L,Westhof E,Yusupov M,Yusupova G

更新日期：2013-06-27 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:Excessive βAR stimulation is an independent factor in inducing pathological cardiac hypertrophy. Here, we report miR-145 regulates both expression and localization of GATA6, thereby protecting the heart against cardiomyocyte hypertrophy induced by isoproterenol (ISO). The protective activity of miR-145 was associated ...

journal_title：FEBS letters

authors： Li R,Yan G,Zhang Q,Jiang Y,Sun H,Hu Y,Sun J,Xu B

更新日期：2013-06-19 00:00:00

abstract：:Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...

journal_title：FEBS letters

authors： Huang CH,Chen YC,Chen CC

更新日期：2013-06-19 00:00:00

abstract：:G-quadruplex ligands DODC and TMPyP4 have different binding modes to quadruplex structure and cause cell proliferation arrest. Here we showed that DODC was more efficient in cell growth inhibition than TMPyP4. Both G-quadruplex ligands induced nuclear-cytoplasmic shuttling and accumulation of TERT in mitochondria. Thi...

journal_title：FEBS letters

authors： Zhuang XY,Yao YG

更新日期：2013-06-05 00:00:00

abstract：:Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are current...

journal_title：FEBS letters

authors： Shin DH,Choi M,Kim K,Bang G,Cho M,Choi SB,Choi G,Park YI

更新日期：2013-05-21 00:00:00

abstract：:The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...

journal_title：FEBS letters

authors： Nguyen TN,You DJ,Kanaya E,Koga Y,Kanaya S

更新日期：2013-05-02 00:00:00

abstract：:MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...

journal_title：FEBS letters

authors： Miao LJ,Huang SF,Sun ZT,Gao ZY,Zhang RX,Liu Y,Wang J

更新日期：2013-05-02 00:00:00

abstract：:Pancreatic islet amyloid is a characteristic feature of type 2 diabetes. The major protein component of islet amyloid is the polypeptide hormone known as islet amyloid polypeptide (IAPP, or amylin). IAPP is stored with insulin in the β-cell secretory granules and is released in response to the stimuli that lead to ins...

journal_title：FEBS letters

authors： Cao P,Marek P,Noor H,Patsalo V,Tu LH,Wang H,Abedini A,Raleigh DP

更新日期：2013-04-17 00:00:00

abstract：:The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...

journal_title：FEBS letters

authors： Berman HM,Coimbatore Narayanan B,Di Costanzo L,Dutta S,Ghosh S,Hudson BP,Lawson CL,Peisach E,Prlić A,Rose PW,Shao C,Yang H,Young J,Zardecki C

更新日期：2013-04-17 00:00:00

abstract：:In the new view, hydrophobic free energy is measured by the work of solute transfer of hydrocarbon gases from vapor to aqueous solution. Reasons are given for believing that older values, measured by solute transfer from a reference solvent to water, are not quantitatively correct. The hydrophobic free energy from gas...

journal_title：FEBS letters

authors： Baldwin RL

更新日期：2013-04-17 00:00:00

abstract：:CREB binding protein (CBP) is an acetyltransferase that plays an important role in many biological processes. Here, we show that Akt phosphorylates CBP at threonine 1871 and suppresses its acetyltransferase activity by impeding the binding of CBP to histone H3, which results in a decrease in lysine K18 acetylation and...

journal_title：FEBS letters

authors： Liu Y,Xing ZB,Zhang JH,Fang Y

更新日期：2013-04-02 00:00:00

abstract：:Spermiogenesis is a dynamic process leading to alterations in cell morphology. In spermiogenesis, the roles of the histone chaperones are largely unknown. Here, I report the unexpected roles of two Nap family proteins, CG5017/Hanabi and nucleosome assembly protein 1 (Nap1) in Drosophila. Hanabi is mainly localized in ...

journal_title：FEBS letters

authors： Kimura S

更新日期：2013-04-02 00:00:00

abstract：:Plants have evolved to cope with fluctuations in water supply by gating their water channels known as aquaporins. During flooding, a rapid drop of cytosolic pH due to anoxia leads to a simultaneous closure of the aquaporins in the plasma membrane. The closing mechanism has been suggested to involve a conserved histidi...

journal_title：FEBS letters

authors： Frick A,Järvå M,Törnroth-Horsefield S

更新日期：2013-04-02 00:00:00

abstract：:The aim of the present research was to analyze the pathways for phosphatidic acid metabolism in purified nuclei from liver. Lipid phosphate phosphatase, diacylglycerol lipase, monoacylglycerol lipase and PA-phospholipase type A activities were detected. The presence of lysophosphatidic acid significantly reduced DAG p...

journal_title：FEBS letters

authors： Gaveglio VL,Pasquaré SJ,Giusto NM

更新日期：2013-04-02 00:00:00

abstract：:Wilms tumor (WT) is a genetically heterogeneous childhood kidney tumor. Several genetic mutations have been identified in WT patients, including inactivation of WTX, somatic stabilizing CTNNB1, and p53 mutations. However, the molecular mechanisms in tumorigenesis remain largely unexplored. Stat3 is a transcription fac...

journal_title：FEBS letters

authors： Cao X,Liu D,Yan X,Zhang Y,Yuan L,Zhang T,Fu M,Zhou Y,Wang J

更新日期：2013-03-18 00:00:00

abstract：:We have explored the mechanisms involved in the facilitation of glutamate release mediated by the activation of kainate receptors (KARs) in the cortex using isolated nerve terminals (synaptosomes). Kainate (KA) produced an increase on glutamate release at 100 μM. The effect of KA was antagonized by NBQX (with AMPA rec...

journal_title：FEBS letters

authors： Rodríguez-Moreno A,Sihra TS

更新日期：2013-03-18 00:00:00

abstract：:Telomerase maintains the ends of eukaryotic chromosomes, and its activity is an important parameter correlating with the proliferative capacity of cells. We have investigated cell cycle-specific changes in telomerase activity using cultures of Desmodesmus quadricauda, a model alga naturally synchronized by light/dark ...

journal_title：FEBS letters

authors： Ševčíková T,Bišová K,Fojtová M,Lukešová A,Hrčková K,Sýkorová E

更新日期：2013-03-18 00:00:00

abstract：:Early B-cell factor 1 (Ebf1) is a transcription factor whose inactivation in all cells results in high bone mass because of an increase in bone formation. This observation suggests Ebf1 may be an inhibitor of osteoblast differentiation. To test this contention, we analyzed Ebf1 pattern of expression and function in os...

journal_title：FEBS letters

authors： Zee T,Boller S,Györy I,Makinistoglu MP,Tuckermann JP,Grosschedl R,Karsenty G

更新日期：2013-03-18 00:00:00

abstract：:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...

journal_title：FEBS letters

authors： He C,Gu Q,Huang M,Xing J,Chen J

更新日期：2013-01-31 00:00:00

abstract：:The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...

journal_title：FEBS letters

authors： Lam BD,Anthony EC,Hordijk PL

更新日期：2013-01-16 00:00:00

abstract：:We studied the molecular mechanism underlying PDT-induced apoptosis-dependent macrophage activation, particularly through NO production. We demonstrate that NO production is initially induced by HSP70 on the apoptotic cell surface, and is further enhanced by macrophage phagocytosis. Additionally, we found that apoptot...

journal_title：FEBS letters

authors： Song S,Zhou F,Chen WR,Xing D

更新日期：2013-01-16 00:00:00

abstract：:It has previously been shown that jasmonic acid affects the ethylene signaling pathway. EIN2 is a central component of ethylene signaling that is downstream of the receptors. EIN2 has previously been shown to be required for ethylene responses. We found that reducing jasmonic acid levels, either mutationally or chemic...

journal_title：FEBS letters

authors： Kim J,Patterson SE,Binder BM

更新日期：2013-01-16 00:00:00

abstract：:Apolipoprotein E (apoE) is predominantly synthesized by astrocytes in the brain. In this study, we investigated the role of apoE in astrocyte apoptosis. We demonstrated that apoE protects astrocytes from hypoxia-induced apoptosis in a dose-dependent manner. Glutamate release from astrocytic cultures is significantly l...

journal_title：FEBS letters

authors： Zhou S,Wu H,Zeng C,Xiong X,Tang S,Tang Z,Sun X

更新日期：2013-01-16 00:00:00

abstract：:Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational...

journal_title：FEBS letters

authors： Bior BK,Ballif BA

更新日期：2013-01-04 00:00:00

abstract：:FixK₂ is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP₁ and ClpXP₁ were purified and tested. FixK...

journal_title：FEBS letters

authors： Bonnet M,Stegmann M,Maglica Ž,Stiegeler E,Weber-Ban E,Hennecke H,Mesa S

更新日期：2013-01-04 00:00:00

abstract：:CCN2 plays a critical role in the development of mesenchymal tissues such as cartilage and bone, and the binding of CCN2 to various cytokines and receptors regulates their signaling.By screening a protein array, we found that CCN2 could bind to fibroblast growth factor receptors (FGFRs) 2 and 3, with a higher affinity...

journal_title：FEBS letters

authors： Aoyama E,Kubota S,Takigawa M

更新日期：2012-12-14 00:00:00

abstract：:14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...

journal_title：FEBS letters

authors： Sluchanko NN,Gusev NB

更新日期：2012-12-14 00:00:00

abstract：:Meprin α and β, members of the astacin family of zinc metalloproteinases, are unique plasma membrane and secreted proteases known to cleave a wide range of biological substrates involved in inflammation, cancer and fibrosis. In this study, we identified proMMP-9 as a novel substrate and show that aminoterminal meprin-...

journal_title：FEBS letters

authors： Geurts N,Becker-Pauly C,Martens E,Proost P,Van den Steen PE,Stöcker W,Opdenakker G

更新日期：2012-12-14 00:00:00

abstract：:The phosphoglucomutases (PGM) Pgm1, Pgm2, and Pgm3 of the yeast Saccharomyces cerevisiae were tested for their ability to interconvert ribose-1-phosphate and ribose-5-phosphate. The purified proteins were studied in vitro with regard to their kinetic properties on glucose-1-phosphate and ribose-1-phosphate. All tested...

journal_title：FEBS letters

authors： Walther T,Baylac A,Alkim C,Vax A,Cordier H,François JM

更新日期：2012-11-30 00:00:00

abstract：:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

journal_title：FEBS letters

authors： Palombo I,Daley DO

更新日期：2012-11-30 00:00:00

abstract：:Although transthyretin (TTR) is expressed in pancreatic alpha (glucagon) cells in the islets of Langerhans, the function of TTR in pancreatic alpha cells remains unknown. In this study, by using TTR knockout (TTR KO) mice, we determined the novel role of TTR in glucose homeostasis. We demonstrated that TTR KO mice evi...

journal_title：FEBS letters

authors： Su Y,Jono H,Misumi Y,Senokuchi T,Guo J,Ueda M,Shinriki S,Tasaki M,Shono M,Obayashi K,Yamagata K,Araki E,Ando Y

更新日期：2012-11-30 00:00:00

abstract：:Metabolic activities of orexin A (OXA) in mature adipocytes are mediated via PI3K/PKB and PPARγ. However, the effects of OXA on preadipocytes are largely unknown. We report here that OXA stimulates the proliferation and viability of 3T3-L1 preadipocytes and protects them from apoptosis via ERK1/2, but not through PKB....

journal_title：FEBS letters

authors： Skrzypski M,Kaczmarek P,Le TT,Wojciechowicz T,Pruszyńska-Oszmalek E,Szczepankiewicz D,Sassek M,Arafat A,Wiedenmann B,Nowak KW,Strowski MZ

更新日期：2012-11-30 00:00:00

abstract：:Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...

journal_title：FEBS letters

authors： Abelein A,Lang L,Lendel C,Gräslund A,Danielsson J

更新日期：2012-11-16 00:00:00

abstract：:The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elo...

journal_title：FEBS letters

authors： Han SY,Lee M,Hong YK,Hwang S,Choi G,Suh YS,Park SH,Lee S,Lee SH,Chung J,Baek SH,Cho KS

更新日期：2012-11-16 00:00:00